RESUMO
We studied the effect of graphene oxide nanoparticles on the differentiation of human dendritic cells and uptake of nanoparticles by these cells in vitro. The objects of the study were mononuclear cells from healthy donors induced into the phenotype of dendritic cells by cytokines (IL-6 and GM-CSF). We used graphene oxide nanoparticles of different sizes functionalized with linear or branched PEG (P-GO or bP-GO) in concentrations of 5 and 25 µg/ml. It was found that graphene oxide nanoparticles did not affect the viability and percentage of dendritic cells in the culture. However, P-GO nanoparticles (25 µg/ml) suppressed the expression of CD83 on the surface of dendritic cells in cultures, thereby suppressing cell differentiation. Dendritic cells internalized P-GO nanoparticles, particles in high concentration were more actively engulfed, but the size of the particles and the type of PEG did not affect the intensity of this process. In general, P-GO nanoparticles in a concentration of 25 µg/ml can regulate differentiation of dendritic cells by suppressing their maturation.
Assuntos
Grafite , Nanopartículas , Diferenciação Celular , Células Dendríticas , Grafite/farmacologia , HumanosRESUMO
The conditions for constructing an immunosorbent reagent for solid-phase NMR analysis were optimized. For this purpose, we increased the area of ââthe sensitized portion of the membrane to fit the relaxometer coil size and added the agent sorption buffer. This provided the penetration of the anti-ligand molecules into the membrane thickness and their uniform distribution.
Assuntos
Técnicas de Imunoadsorção , Membranas Artificiais , Ressonância Magnética Nuclear BiomolecularRESUMO
The effects of chorionic gonadotropin (hCG) on the expression of the hTERT gene in combination with the conversion of the phenotype of naive T-cells and T-cells of immune memory in vitro were studied. hCG inhibited expression of hTERT mRNA in naive T-cells (CD45RA+) and immune memory T cells (CD45RO+), causing a decrease in the replicative potential of the cells. The presence of hCG in the culture led to the conversion of the phenotype of T-lymphocytes. hCG reduced the number of proliferating T-cells of immune memory, estimated by phenotypic signs by differential gating. hCG (10 IU/ml and 100 IU/ml) inhibited expression of CD25 by the studied populations, but did not modulate expression of the CD71 proliferation marker. Thus, hCG inhibited the functional activity of naive T-cells and T-cells of immune memory, which, in the context of pregnancy, can contribute to the formation of immune tolerance to the semi-allogenic fetus.
Assuntos
Diferenciação Celular , Gonadotropina Coriônica/fisiologia , Linfócitos T/citologia , Telomerase/metabolismo , Células Cultivadas , Feminino , Humanos , Antígenos Comuns de Leucócito/metabolismo , Fenótipo , Gravidez , Telomerase/genéticaRESUMO
The effect of native α-fetoprotein (AFP) on the expression of T-regulatory lymphocyte (Treg) markers by activated CD4+ lymphocytes with different proliferative status was studied. α-Fetoprotein did not affect the ratio of proliferating and non-proliferating activated CD4+ cells. In the study of Treg differentiation, it was found that AFP at concentrations of 50 and 100 µg/mL significantly inhibited the number of nonproliferating CD4+FOXP3+ and CD4+FOXP3+HELIOS+ lymphocytes without affecting the expression of Treg markers by proliferating CD4+ lymphocytes.